Poliovirus Receptor Protein (PVR) (C-Term, Extracellular Domain)

Catalog No. ABIN1684720

This Recombinant Poliovirus Receptor protein is produced in HEK-293T Cells.

Quick Overview for Poliovirus Receptor Protein (PVR) (C-Term, Extracellular Domain) (ABIN1684720)

Target: Poliovirus Receptor (PVR)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293T Cells

Purity: > 95 %, as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: C-Term, Extracellular Domain

Specificity: Optimized DNA sequence encoding extracellular domain of rat PVR (CD155) including a C-terminal 6His tag was expressed in HEK293 cells.

Characteristics: Recombinant rat PVR (CD155) is a monomer protein consisting of 329 amino acid residue subunits, due to glycosylation migrates as an approximately 55-60 kDa protein on SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Application Details

Comment:

The biological activity of rat PVR was measured by the binding ability of immobilized recombinant rat CD155 (20 µg/mL100 µL/well ) to mouse CD226 (linear ranger of 6 - 200 ng/mL) in a functional ELISA assay.

Restrictions: For Research Use only

Handling

Buffer: PBS solution, pH7.2

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this protein can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Expiry Date: 12-24 months

Target Details

Target: Poliovirus Receptor (PVR)

Alternative Name: CD155

UniProt: Q5U334

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome